Citations (93) of Sergei. These publications cited papers and books of a Moscow University researcher Sergei Ostroumov (environmental science, biology). Time interval up to 2011, selected examples.
http://5bio5.blogspot.com/2016/07/citations-93-of-sergei.htmlSource: http://libra.msra.cn/Detail?searchtype=5&entitytype=2&id=54959579&start=91&end=100
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- Flood mitigation designs with respect to river ecosystem functions—A problem oriented conceptual approach (Citations: 3)
We formulated five proposals to improve specifications of flood hazard reduction projects and five proposals to preserve the river ecosystem functions, based on literature and authors’ experience. Starting from these, we established a conceptual approach using the notion of riverscape, with related function richness and bioassessment tools. We defined four riverscape types based on the gradient of artificialness, from type ...
For the first time the potential of Noctiluca scintillans, a non-toxic mixotrophic dinoflagellate, in bioconverting and/or excreting saxitoxin has been illustrated, thus contributing to the limited knowledge on the aspects of toxin pathways in the food chain/web and predator-prey preferences. Noctiluca growth rate increased with higher Pyrodinium concentration but the ratio of Noctiluca to Pyrodinium should ...
Journal: Toxicon , vol. 55, no. 5, pp. 1017-1023, 2010 Sponges can filter large amounts of water, which exerts an important grazing impact on free bacteria, an important component of the diet of sponges. We examined the accumulation of bacteria in the Demospongiae (Hymeniacidon perlevis). Analyses were performed on homogenates from unstarved and starved sponges in seawater from their sampling site (the Ionian Sea). Culturable heterotrophic bacteria (22°C), total culturable ...
In order to find the segments of Pinios River which lack the retention capacity of the BOD and nutrient input, the difference of the estimated input and output pollution loads was compared at upstream and downstream clustered areas of a total of 73 segments. Catchment areas ranged from 1 to 11,300km2. Emissions were always higher than the actual transport, ...
S. A. Ostroumov, G. M. Kolesov Ostroumov S. A., Kolesov G. M. The aquatic macrophyte Ceratophyllum demersum immobilizes Au nanoparticles after their addition to water. - Doklady Biological Sciences, 2010 (Mar - Apr), Vol. 431, p. 124–127. [© Pleiades Publishing, Ltd., ISSN 0012-4966]. Original Russian Text © S.A. Ostroumov, G.M. Kolesov, 2010, published in Doklady Akademii Nauk, 2010, Vol. 431, No. 4, pp. 566–569. Bibliogr. ...
S. A. Ostroumov, G. M. Kolesov Biodetritus that formed over a 7-month period in microcosms with Viviparus viviparus, Unio pictorum, and Ceratophyllum demersum contained a number of elements. This is the first study to determine concentrations of some elements in biodetritus. Neutron activation analysis showed that the elements’ concentrations decrease in the following order: Ca > Zn > Ba > Br > Ce > Se &...
The effects of in vivo exposure of Mytilus galloprovincialis to two anionic surfactants (SDBS and SDS) on the molecular biomarker system were studied. After continuous exposure for 72 days, activities/levels of GST, GPx and GSH were significantly higher than in corresponding control groups following exposure to 3.000mg/L SDS and SDBS. Activities of SOD and CAT were significantly ...
Biological ion transport processes in proteins have inspired the development of bio-sensors, actuators, photoelectric, and chemoelectric energy conversion devices. These bio-inspired devices use ion transport through a protein energized by biochemical reactions in the protein’s sub-units to perform their engineering function. In an effort to advance the use of biological processes in synthetic systems, a chemoelectrical energy ...
Along with the sequent completion of Manwan and Dachaoshan Dam, the river continuum of the middle and lower reaches of the Lancang River was separated into three types of segments: reservoir, below-dam segment and downstream flowing segment. The long-term series of water quality and river flow data over 20 years were analyzed in order to study the impact of ...
E. V. Lazareva, S. A. Ostroumov A new contribution to the scientific basis of phytoremediation and phytotechnology for water treatment. It is the first paper that reports the experiment in which it was proved that the aquatic plants helped remove a synthetic surfactant from water. ** It was discovered that plant biomass help towards restoration the water quality in the system which was polluted with a synthetic ...
The concept of critical nutrient amounts (CNA) for a watershed was developed to address eutrophication in surface waters from diffuse (non-point) source pollution. CNA is defined as the maximum allowable applied or generated amount (AGA) of a nutrient from natural and human sources that can be emitted and still allow compliance with water quality standards. The CNA calculation method ...
S. A. Ostroumov, T. V. Shestakova . In 1950 s I started in science by showing that non-phosphorylating respiration is critical for survival of an animal at low temperature. Later, in the 1960 s and 1970 s, I took part in verification of Mitchell’s chemiosmotic hypothesis postulating that (i)mitochondria transform energy of respiration to electricity and (ii) uncoupling of respiration represents discharge of this electricity ...
Journal: Experientia , vol. 66, no. 11, pp. 1785-1793, 2009 The kinetics of the formation and relaxation of transmembrane electric potential (Δψ) during the complete single turnover of CcO was studied in the bovine heart mitochondrial and the aa3-type Paracoccus denitrificans enzymes incorporated into proteoliposome membrane. The real-time Δψ kinetics was followed by the direct electrometry technique. The prompt oxidation of CcO and formation of the activated, oxidized (...
I. M. Vorozhun, S. A. Ostroumov Many species of planktonic and benthic invertebrates are active filter-feeders. The filtration activity of aquatic organisms is of great importance for the functioning of ecosystems [1‐3]. It was shown earlier that surfactant sodium dodecylsulfate (SDS) inhibits the filtration activity of Mytilus edulis, M. galloprovincialis , and some other aquatic filter-feeders [4‐15], which is manifested in a decreased removal of ...
Freshwater mussel (Superfamily Unionoidea) communities are important components of food webs, and they link and influence multiple trophic levels. Mussels filter food from both the water column and sediment with ciliated gills. Differences in cilia structure and arrangement might allow mussel species to partition food resources. Mussels are omnivores that feed across trophic levels on bacteria, algae, detritus, zooplankton, and ...
This study was carried out to evaluate the microbiological accumulation capability of the demosponge Spongia officinalis var. adriatica. Six microbiological parameters were researched in two sampling periods in the water and in reared sponge samples coming from sites with different degrees of microbial contamination: an off-shore fish farm displaced off the Apulian coast (Southern Adriatic Sea) and a no-...
Abstract A Bilevel Optimization Algorithm to Identify Enzymatic Capacity Constraints in Metabolic Networks–Development and Application Laurence Yang
Published in 2008.
A number of the electrogenic reactions in photosystem I, photosystem II, and bacterial reaction centers (RC) were comparatively analyzed, and the variation of the dielectric permittivity (ε) in the vicinity of electron carriers along the membrane normal was calculated. The value of ε was minimal at the core of the complexes and gradually increased towards the periphery. We found that ...
The kinetics of the oxidation of fully-reduced ba3 cytochrome c oxidase from Thermus thermophilus by oxygen were followed by time-resolved optical spectroscopy and electrometry. Four catalytic intermediates were resolved during this reaction. The chemical nature and the spectral properties of three intermediates (compounds A, P and O) reproduce the general features of aa3-type oxidases. However the F ...
Nitric oxide reductase (NOR) from P. denitrificans is a membrane-bound protein complex that catalyses the reduction of NO to N2O (2NO+2e−+2H+→N2O+H2O) as part of the denitrification process. Even though NO reduction is a highly exergonic reaction, and NOR belongs to the superfamily of O2-reducing, proton-pumping heme-copper oxidases (HCuOs), previous measurements have indicated that the reaction catalyzed ...
The present study investigates the effect of the filtering activity of Sabella spallanzanii on the culturable heterotrophic bacterial community through the comparison of the bacterial diversity in transplanted polychaetes and the surrounding seawater. For isolation of culturable heterotrophic bacteria, seawater samples as well as polychaete homogenates were plated in triplicates onto Bacto Marine Agar 2216 (Difco). All the colonies grown ...
Mechanisms of enzymatic decomposition of an organic substance consisting of fractal macromolecules of different sizes are studied. A kinetic decomposition equation in which the reaction rate coefficient depends on the characteristic of the fractal structure is formulated. The solution for a fraction of macromolecules of the same size and for a mixture of macromolecules of different sizes is analyzed. It ...
The expression of protein biomarkers in Baltic Sea blue mussels was analyzed after three days exposure to low (2.8 μg/animal/day), intermediate (28 μg/animal/day), or high (280 μg/animal/day) nominal doses of benzo[a]pyrene (BaP). Significant expression changes were found in the animals exposed to the low dose, the lowest reported dose for DNA adduct formation in ...
Suspension-feeders are discriminate feeders, selecting prey by size, shape and food quality, this discriminate feeding behaviour has important consequences for the modelling of growth rates, population dynamics and ecosystem change. When given natural seston, the large pinnid bivalve Atrina zelandica (Gray), fed on picophytoplankton (<2 μm), larger phytoplankton (2–270 μm) and microzooplankton, but preferentially selected algal species within the ...
In arid and semi-arid regions of the southwestern United States and other parts of the world, flows of historically ephemeral streams are now perennially dominated by municipal and/or industrial effluent discharges, particularly in urbanized watersheds. Because effluent-dominated and dependent water bodies have previously received limited scientific study, we reviewed select contemporary topics associated with water quality of ...
A study on the filtering activity has been carried out on reared specimens of the demosponge Spongia officinalis var. adriatica coming from an off-shore farm displaced off the Apulian coast (Ionian Sea). The experience was carried out under laboratory conditions, by using natural seawater collected from the sponge environment. The study demonstrates a high efficiency of the sponge in ...
Pollution resulting from increased human activities is threatening Lake Donghu, its effects being characterized by serious eutrophication. A steady increase of phosphorus loading is the most important factor of the lake eutrophication. Pollution external control projects are being implemented and will be accomplished before the year 2010. In order to predict the restoration rate by the lake's self-purification ...
Proton translocation in the catalytic cycle of cytochrome c oxidase (CcO) proceeds sequentially in a four-stroke manner. Every electron donated by cytochrome c drives the enzyme from one of four relatively stable intermediates to another, and each of these transitions is coupled to proton translocation across the membrane, and to uptake of another proton for production of water in ...
The potential of the red agarophyte Gracilariopsis longissima as biofilter for phosphate and ammonium in effluents outflowing intensive marine fish cultures was assessed at different scales. Previous studies showed that both laboratory (microscale level) and outdoor cultivation (mesoscale level) were feasible, with a maximum sustainable yield of 270 g fresh wt m−2 day−1 approximately, at a biomass higher than that ...
Journal: Aquaculture , vol. 252, no. 1, pp. 43-53, 2006 The recent discovery of a carotenoid light-harvesting antenna in xanthorhodopsin, a retinal-based proton pump in Salinibacter ruber, made use of photoinhibition of respiration in whole cells to obtain action spectra [Balashov et al. Science 309, (2005) 2061–2064]. Here we provide further details of this phenomenon, and compare action spectra in three different systems where carotenoids have different ...
S. A. Ostroumov, J. Widdows Effects of three surfactants on the filtration rates by marine mussels were studied. The xenobiotics tested represented anionic, cationic and non-ionic surfactants (tetradecyltrimethylammonium bromide, a representative of a class of cationic surfactants; sodium dodecyl sulphate, a representative of anionic alkyl sulfates; and Triton X-100, a representative of non-ionic hydroxyethylated alkyl phenols). All three surfactants inhibited the clearance ...
Published in 2006.
S. A. Ostroumov
On the basis of the previous publications, our new data and the existing scientific literature, we have formulated some fundamental principles that characterize the pivotal roles of the biodiversity of filter-feeders in ecosystems. Among those roles are: (1) the role of ecological repair of water quality, (2) the role of contributing to reliability and stability of the functioning of ...
Purple membranes were adsorbed on freestanding lipid bilayers, termed nano-black lipid membranes (nano-BLMs), suspending the pores of porous alumina substrates with average pore diameters of 280nm. Nano-BLMs were obtained by first coating the upper surface of the highly ordered porous alumina substrates with a thin gold layer followed by chemisorption of 1,2-dipalmitoyl-sn-glycero-3-...
An integrated outdoor cultivation of two macroalgal species: Ulva rotundata (Chlorophyta) and Gracilariopsis longissima (Rhodophyta) was designed. The macroalgae were cultured in effluents from an intensive marine culture (growout phase) of gilthead seabream Sparus aurata. The biomass evolution of the algal tanks followed a logistic curve, where the approach to the maximum stocking density of seaweeds was governed by thalli ...
A nonlinear model of the decomposition of a multicomponent contaminant is developed with allowance made for the distribution of components over their reactivity. Model application is illustrated by the analysis of published empirical data on organic matter biodegradation in water column and bottom sediments, in an activated-sludge bioreactor, and the biological absorption and enzymatic destruction of organic matter by ...
S. A. Ostroumov
Principles of the theory of the ecological mechanism of water self-purification based on multiple functions of the biota in freshwater and marine ecosystems are formulated. In developing this theory, the results of the author's experiments with filtering hydrobionts have been used. These results indicate that the water self-purification mechanism is vulnerable to the impact of some pollutants ...
S. A. Ostroumov
Generalizations presented in this paper represent, in systematized form, the basic elements of the qualitative theory of water self-purification in freshwater and marine ecosystems. Recommendations are given for maintaining water quality and sustainable development of water resources. Results of experimental studies of the effect exerted by Triton X-100 and OMO synthetic detergent on mollusks Unio tumidus.
S. A. Ostroumov
Ostroumov S.A. Some aspects of water filtering activity of filter-feeders // Hydrobiologia. 2005. Vol. 542, No. 1. P. 275 – 286. www.scribd.com/doc/44105992/; On the basis of the previous publications, our new data and the existing scientific literature, we have formulated some fundamental principles that characterize the pivotal roles of the biodiversity of filterfeeders in ecosystems. Among ...
S. A. Ostroumov
Long-term studies of the biological effect of surfactants, including the effect surfactants exert on filter feeders, are reviewed. The role of filter feeders in the functioning of freshwater and marine ecosystems is analyzed. New aspects in the assessment of environmental hazard due to the impact of chemical pollutants, including surfactants and detergents, are established.
Transient electrical currents generated by the Na+-transporting FoF1-ATPase of Ilyobacter tartaricus were observed in the hydrolytic and synthetic mode of the enzyme. Two techniques were applied: a photochemical ATP concentration jump on a planar lipid membrane and a rapid solution exchange on a solid supported membrane. We have identified an electrogenic reaction in the reaction cycle of the ...
S. A. Ostroumov
Effects of several surfactants and chemical mixtures on marine bivalves were studied. An anionic surfactant, sodium dodecylsulphate (SDS), and a cationic surfactant, tetradecyltrimethylammonium bromide (TDTMA), inhibited the filtering activity of oysters (Crassostrea gigas). Similar effects were exhibited by some chemical mixtures that included surfactants. Those mixtures inhibited the filtering activity of Crassostrea gigas and Mytilus galloprovincialis. The new results are ...
Transmembrane ion pumps are often investi- gated experimentally by photoelectric measurements in model systems. In addition to the most widely used systems based on model membranes, a fundamentally different class is represented by the so-called suspension methods. In this technique the electric signal is measured on a bulk suspension of oriented ion pumps in the form of a displacement ...
The striking properties of monolayers and multilayers of photosensitive proteins obtained by using the Langmuir-Blodgett technique are described. The close packing of the protein molecules, which preserve most of the properties found in solution, seems to be the main cause for their thermal stability, which in some cases reached a temperature of 200°C without the loss of the protein ...
Discusses the fundamental principles of the fast photoelectric effect and its applications to molecular electronics research. The principles are illustrated with experimental data obtained from reconstituted bacteriorhodopsin membranes. The following topics are considered: Halobacterium halobium; light-induced charge separation; equivalent circuit analysis; the concept of intelligent materials; reverse engineering of visual rhodopsin and bacteriorhodopsin; bacteriorhodopsin as an advanced bioelectronic material; ...
Summary Membrane fragments containing the H+K--ATPase from parietal cells have been adsorbed to a planar lipid membrane. The transport activity of the enzyme was determined by measuring electrical currents via the capacitive coupling between the membrane sheets and the planar lipid film. To initiate the pump currents by the ATPase a light-driven concentration jump of ATP from caged ...
Summary Purified ATP synthase (F0F1) fromRhodospirillum rubrum was reconstituted into asolectin liposomes which were than adsorbed to a planar lipid bilayer. After the addition of an inactive photolabile ATP derivative (caged ATP), ATP was released after illumination with UV light, which led to a transient current in the system. The transient photocurrent indicates that the vesicles and the planar membrane ...
Summary Nonstationary electric currents are described which are generated by the Na,K-pump. Flat membrane sheets 0.2–1 µm in diameter containing a high density of oriented N,K-ATPase molecules are bound to a planar lipid bilayer acting as a capacitive electrode. In the aqueous phase adjacent to the bound membrane sheets, ATP is released within milliseconds ...
Many natural and synthetic compounds are used in studying the relationships between semiautonomous cellular organelles and nueleocytoplasm. These studies, together with analyses of the organelle macromolecules by methods of molecular biology, constitute a firm basis for adoption of the endosymbiotic origin of plastids and mitochondria.
Ca++-ATPase activity was studied ultracytochemically (cf. Ando et al. 1981) in the paraphysis cerebri of the frog. An intense reaction was demonstrated on the plasmalemma of the microvilli at the apical pole of paraphyseal cells; in contrast, the basolateral plasmalemma showed only a slight staining. In addition, mitochondria, gap junctions, cilia, and cytoplasmic elements (e.g., microfilaments) displayed Ca++-...
Summary A new observation on theH. halobium cells is reported. It has been observed that when the cells are exposed to light a volume flow is observed through them. The magnitude of the light-induced volume flow depends on the intensity and wavelength of the exciting light and is also influenced by temperature. The phenomenon appears to be relevant to ...
Journal: Experientia , vol. 40, no. 7, pp. 773-775, 1984
In order to determine the attachment site(s) of the chromophore retinal in bacteriorhodopsin and to examine the possible occurrence of light-induced changes in its binding site, all accessible lysine residues in bacteriorhodopsin were modified by reductive methylation with formaldehyde and NaCNBH3. Upon transformation of the ?-amino group of the lysyl residues to the ?-N-dimethyl derivatives, these amino ...
Summary Bacteriorhodopsin-mediated photopotential generation has been studied in two kinds of lipid/water systems: (1) decane solution of asolectin was used as the lipid phase; (2) a mixture of bacteriorhodopsin sheets and hexane solution of phosphatidyl choline was applied onto a water surface to form a monolayer, and then the monolayer was covered with a 0.3-mm decane ...
Vacuoles isolated from storage roots of red beet (Beta vulgaris L.) posess a Mg2+-dependent, alkaline pyrophosphatase (PPase) activity which is further stimulated by salts of monovalent cations. The requirement for Mg2+ is specific. Mn2+ and Zn2+ permitted only 20% and 12%, respectively, of the PPase activity obtained in the presence of Mg2+ while Ca2+, Co2+ and Cu2+ were ineffective. ...
Journal: Planta , vol. 153, no. 2, pp. 150-155, 1981 Light-induced fluorescence changes of 9-aminoacridine, an indicator of proton gradient in energy-transducing membranes, were studied in Plectonema boryanum and other cyanobacteria. The fluorescence changes observed in cell suspensions resulted from a superposition of fluorescence quenching and enhancement as the analysis of the kinetic data shows. Both components of the fluorescence changes are abolished by 3-(3,4-...
A S S X R A C T Bacteriorhodopsin, in known amounts and controlled orientation, is incorporated into planar membrane films. These films are formed by the sequential transfer of two air-water interface films onto a thin, hydrophilic, electrically conductive support cast from nitrocellulose. The films are easily accessible to electrical measurements and to control of the ionic milieu ...
When purple-membrane fragments from Halobacterium halobium are added to one aqueous phase of a positively-charged black lipid membrane, the membrane becomes photoelectrically active. Under normal conditions the steady-state photo-current is extremely low, but increases considerably when the lipid bilayer is doped with proton-permeable gramicidin channels or with a lipophilic acid-base system. These findings indicate ...
Summary Purple membrane vesicles prepared by different techniques differ widely in their morphology and ability to establish a proton gradient in the light. The procedures used to prepare active vesicles do not completely dissociate the purple membrane and thus preserve a preferential orientation of the protein, while most of the lipid is exchanged for added lipid. Responses to illumination are ...
A photosensory mechanism is proposed for Halobacterium halobium based on the observation of light-induced motor responses. Possible mechanisms of signal transduction in Halobacterium are discussed. Bacteriorhodopsin and the visual pigment rhodopsin are compared with respect to their structural and functional properties. The conclusion is drawn that Halobacterium may help to understand primary photochemical events of rhodopsin rather than the ...
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Summary Interface films of purple membrane and lipid containing spectroscopically intact and oriented bacteriorhodopsin have been used as a model system to study the function of this protein. Small positive charges in surface potential (<1 mV) are detected upon illumination of these films at the air-water interface. These photopotentials, are not affected by overlaying the interface film with ...
Some basic aspects of incorporation of hydrophobic peptides and proteins in artificial lipid membranes are discussed. As examples valinomycin as a carrier model and gramicidin A as a channel former in lipid vesicles and in planar lipid membranes are presented.
Summary Addition of fragmented sarcoplasmic reticulum (SR) vesicles to the aqueous phase of a black lipid membrane (BLM) causes a large increase in BLM conductance within 10 min. The conductance increase is absolutely dependent on three conditions: The presence of at least 0.5mm Ca++, an acidic phospholipid such as phosphatidylserine or diphosphatidylglycerol in the BLM phospholipid mixture, and an ...
Halobacterium halobium contains a retinal-protein complex, bacteriorhodopsin, which is the only protein of the so-called purple membrane forming distinct patches within the surface membrane1,2. Bacteriorhodopsin acts as a photoreceptor molecule and is chemically similar to the visual pigment rhodopsin1,3. On illumination bacteriorhodopsin undergoes a fast cyclic photoreaction with at least four intermediates occurring after microseconds and ...
Journal: Nature , vol. 257, no. 5521, pp. 46-48, 1975 Photoelectric methods to study charge transfer processes in photosynthetic organisms are reviewed along with comparative analyses of photoelectric reactions in Photosystem I, Photosystem II, and the reaction center of purple bacteria. Particular emphasis is placed on a comparison of dielectrically weighted photoelectric signal amplitudes with local structural parameters obtained from the X-ray diffraction data.
Organismal modifications to their physical and chemical environment play a significant role in structuring many modern ecosystems, and experimental evidence suggests that such behavior can increase diversity. Despite the important role such activities play in connecting ecology and evolution, less is known of the macroevolutionary impact of such influences, especially their role during major evolutionary transitions. The Ediacaran-Cambrian diversification ...
Sergei A. Ostroumov
New data on effects of chemicals (surfactants) on water filtration by aquatic invertebrates are reported. The basics of the new theory of the polyfunctional role of biota in self-purification of water in aquatic ecosystems (lakes, rivers, man-made reservoirs) are formulated. The theory covers the following: sources of energy for the mechanisms of self-purification; the main functional blocks ...
In this chapter, the combined effects of eutrophication and of heavy metal contamination on the zooplankton community of a freshwater ecosystem are analyzed. Through biomonitoring, it was possible to study zooplanktonic attributes as indicators of environmental stress: species richness, species diversity, equity, and biomass. These attributes allowed the detection of structural and functional changes. There was an inverse relationship between ...
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