Italy - University of Palermo, University of Messina, Consorzio Universitario della Provincia di Trapani, Istituto di Biologia marina, -
New citation of Moscow University research results (this paper was cited: Ostroumov 2003), ecology, marine ecotoxicology: tags: aninonic surfactant, Sodium Dodecyl Sulfate, SDS, Mediterranean Mussel, Mytilus Galloprovincialis, biomarkers
Италия - итальянские ученые - цитирование научных публикаций МГУ.
http://5bio5.blogspot.com/2014/10/italy-new-citation-of-moscow-university_88.html
Effect Of Sodium Dodecyl Sulfate (Sds) On Stress Response In The Mediterranean Mussel (< i> Mytilus Galloprovincialis</i>): Regulatory Volume Decrease (Rvd) And …
CM Messina, C Faggio, VA Laudicella, M Sanfilippo… - Aquatic Toxicology, 2014;
Abstract In this study the effects of an anionic surfactant, sodium dodecyl sulfate (SDS), are
assessed on the Mediterranean mussel (Mytilus galloprovincialis), exposed for 18 days at a
concentration ranging from 0.1 mg/l to 1 mg/l. The effects are monitored using biomarkers ...
**
Aquatic Toxicology
Available online 13 October 2014
In Press, Accepted Manuscript — Note to users
Effect Of Sodium Dodecyl Sulfate (Sds) On Stress Response In The Mediterranean Mussel (Mytilus Galloprovincialis): Regulatory Volume Decrease (Rvd) And Modulation Of Biochemical Markers Related To Oxidative Stress
Concetta Maria Messina a, 1, , ,
Caterina Faggio b, 1,
Vincenzo Alessandro Laudicella c,
Marilena Sanfilippo b,
Francesca Trischitta b,
Andrea Santulli a, c
they cited paper: Ostroumov, 2003 (Moscow University):
.. discharge of anionic surfactants has increased the awareness on the effects of these compounds
on antioxidant system of bivalves and fish (Alvarez-Munoz et al., 2009, da Silva and Meirelles,
2004, Guilhermino et al., 2000, Nunes et al., 2008, Ostroumov, 2003, Romanelli et al ...
**
Abstract
In this study the effects of an anionic surfactant, sodium dodecyl sulfate (SDS), are assessed on the Mediterranean mussel (Mytilus galloprovincialis), exposed for 18 days at a concentration ranging from 0.1 mg/l to 1 mg/l. The effects are monitored using biomarkers related to stress response, such as regulatory volume decrease (RVD), and to oxidative stress, such as reactive oxygen species (ROS), endogenous antioxidant systems and Hsp70 levels. The results demonstrate that cells from the digestive gland of M. galloprovincialis, exposed to SDS were not able to perform the RVD owing to osmotic stress. Further, SDS causes oxidative stress in treated organisms, as demonstrated by the increased ROS production, in comparison to the controls (p < 0.05). Consequently, two enzymes involved in ROS scavenging, superoxide dismutase (SOD) and catalase (CAT) have higher activities and the proportion of oxidized glutathione (GSSG) is higher in hepatopancreas and mantle of treated animals, compared to untreated animals (p < 0.05). Furthermore Hsp70 demonstrates an up-regulation in all the analysed tissues of exposed animals, attesting the stress status induced by the surfactant with respect to the unexposed animals. The results highlight that SDS, under the tested concentrations, exerts a toxic effect in mussels in which the disruption of the osmotic balance follows the induction of oxidative stress.
Key-words
Mytilus galloprovincialis;
SDS (sodium dodecyl dulfate);
cell volume regulation;
biomarkers of oxidative stress;
endogenous antioxidants
Corresponding author. Laboratorio di Biochimica Marina ed Ecotossicologia (LaBMEco). Via Barlotta, 4 Trapani, 91100; Tel.: +39 0923560162; fax: +39 09160666.
**
New citation of Moscow University research results (this paper was cited: Ostroumov 2003), ecology, marine ecotoxicology: tags: aninonic surfactant, Sodium Dodecyl Sulfate, SDS, Mediterranean Mussel, Mytilus Galloprovincialis, biomarkers
Италия - итальянские ученые - цитирование научных публикаций МГУ.
http://5bio5.blogspot.com/2014/10/italy-new-citation-of-moscow-university_88.html
Effect Of Sodium Dodecyl Sulfate (Sds) On Stress Response In The Mediterranean Mussel (< i> Mytilus Galloprovincialis</i>): Regulatory Volume Decrease (Rvd) And …
CM Messina, C Faggio, VA Laudicella, M Sanfilippo… - Aquatic Toxicology, 2014;
Abstract In this study the effects of an anionic surfactant, sodium dodecyl sulfate (SDS), are
assessed on the Mediterranean mussel (Mytilus galloprovincialis), exposed for 18 days at a
concentration ranging from 0.1 mg/l to 1 mg/l. The effects are monitored using biomarkers ...
**
Aquatic Toxicology
Available online 13 October 2014
In Press, Accepted Manuscript — Note to users
Effect Of Sodium Dodecyl Sulfate (Sds) On Stress Response In The Mediterranean Mussel (Mytilus Galloprovincialis): Regulatory Volume Decrease (Rvd) And Modulation Of Biochemical Markers Related To Oxidative Stress
Concetta Maria Messina a, 1, , ,
Caterina Faggio b, 1,
Vincenzo Alessandro Laudicella c,
Marilena Sanfilippo b,
Francesca Trischitta b,
Andrea Santulli a, c
- a University of Palermo, Department of Earth and Marine Science DiSTeM, Marine Biochemistry and Ecotoxicology Laboratory, Via G. Barlotta 4, 91100, Trapani, Italy
- b Department of Biological and Environmental Science, University of Messina, Viale Ferdinando Stagno d’Alcontres 31, 98166 Messina, Italy
- c Consorzio Universitario della Provincia di Trapani, Istituto di Biologia marina, Via G. Barlotta 4, 91100, Trapani, Italy
they cited paper: Ostroumov, 2003 (Moscow University):
.. discharge of anionic surfactants has increased the awareness on the effects of these compounds
on antioxidant system of bivalves and fish (Alvarez-Munoz et al., 2009, da Silva and Meirelles,
2004, Guilhermino et al., 2000, Nunes et al., 2008, Ostroumov, 2003, Romanelli et al ...
**
Abstract
In this study the effects of an anionic surfactant, sodium dodecyl sulfate (SDS), are assessed on the Mediterranean mussel (Mytilus galloprovincialis), exposed for 18 days at a concentration ranging from 0.1 mg/l to 1 mg/l. The effects are monitored using biomarkers related to stress response, such as regulatory volume decrease (RVD), and to oxidative stress, such as reactive oxygen species (ROS), endogenous antioxidant systems and Hsp70 levels. The results demonstrate that cells from the digestive gland of M. galloprovincialis, exposed to SDS were not able to perform the RVD owing to osmotic stress. Further, SDS causes oxidative stress in treated organisms, as demonstrated by the increased ROS production, in comparison to the controls (p < 0.05). Consequently, two enzymes involved in ROS scavenging, superoxide dismutase (SOD) and catalase (CAT) have higher activities and the proportion of oxidized glutathione (GSSG) is higher in hepatopancreas and mantle of treated animals, compared to untreated animals (p < 0.05). Furthermore Hsp70 demonstrates an up-regulation in all the analysed tissues of exposed animals, attesting the stress status induced by the surfactant with respect to the unexposed animals. The results highlight that SDS, under the tested concentrations, exerts a toxic effect in mussels in which the disruption of the osmotic balance follows the induction of oxidative stress.
Key-words
Mytilus galloprovincialis;
SDS (sodium dodecyl dulfate);
cell volume regulation;
biomarkers of oxidative stress;
endogenous antioxidants
Corresponding author. Laboratorio di Biochimica Marina ed Ecotossicologia (LaBMEco). Via Barlotta, 4 Trapani, 91100; Tel.: +39 0923560162; fax: +39 09160666.
**